Low Pressure Microfluidic-based Dna Fragmentation
نویسندگان
چکیده
We report a low-pressure microfluidic deoxyribonucleic acid (DNA) fragmentation device based on a combination of mechanical hydrodynamic shearing and low temperature sample heating. Conventional DNA fragmentation based on hydrodynamic shearing is capable of achieving fragment lengths (FL) < 10k bp with pressures ∆P > 1 bar. The application of a small amount of additional thermal energy to the buffer medium results in ~10x reduction in pressure requirement resulting in FL~6k-8k bp with ∆P~1-0.1 bar with sample temperature Ts~45o C
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